Multiplexed Sequence Encoding: A Framework for DNA Communication Work is in progress to clone this gene into an adequate host to study the specific role and possible biotechnological applications of this alternative peroxidase source. Sequence alignment showed that only three peroxidases have a significant identity with Bn PA namely AtP29a (84%), and At PA2 (81%) from Arabidopsis thaliana, and HRPA2 (82%) from horseradish (Armoracia rusticana). The putative peroxidase (Bn PA) showed a calculated Mr of 34kDa, and isoelectric point (pI) of 4.5, with no significant identity with other reported turnip peroxidases. The full length c DNA is 1167bp long and contains a 1077bp open reading frame (ORF) encoding a 358 deduced amino acid peroxidase polypeptide. Two c DNA fragments were purified, sequenced, and aligned with the partial sequence from RT-PCR, and a complete overlapping sequence was obtained and labeled as Bb PA (Genbank Accession No. The resulting partial sequence was used to design the rest of the specific primers for 5' and 3' RACE. Total RNA extracted from mature turnip roots was used as a template for RT-PCR, using a degenerated primer designed to amplify the highly conserved distal motif of plant peroxidases. purple top white globe) by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of c DNA ends (RACE). Romero-Gómez, S Duarte-Vázquez, M A GarcÃa-Almendárez, B E Mayorga-MartÃnez, L Cervantes-Avilés, O Regalado, CĪ putative peroxidase c DNA was isolated from turnip roots (Brassica napus L. All rights reserved.Ī putative peroxidase c DNA from turnip and analysis of the encoded protein sequence. This brief review summarizes recent advances that have been made on the encoding strategies for DNA-templated libraries, and it also highlights their respective advantages and limitations for the preparation of DNA-encoded libraries. Li, Gang Zheng, Wenlu Liu, Ying Li, XiaoyuĪmong various types of DNA-encoded chemical libraries, DNA-templated library takes advantage of the sequence-specificity of DNA hybridization, enabling not only highly effective DNA-templated chemical reactions, but also high fidelity in library encoding. Novel encoding methods for DNA-templated chemical libraries. The invention further provides p95 knock-out mice. Also provided are methods of detecting p95 and DNA encoding p95. Morgan, William Francis Maser, Richard Scott Carney, James PatrickĪn isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. These studies on DNA polymerase encoded by PaP1 help advance our knowledge on phage- encoded DNA polymerases and elucidate PaP1 propagation in infected P. The optimized conditions for polymerization were a temperature of 37 Â☌ and a buffer consisting of 40 mM Tris-HCl (pH 8.0), 30 mM MgCl2, and 200 mM NaCl. Gp90 can degrade ss DNA and ds DNA in 3'-5' direction at a similar rate, which is considerably lower than that of T7 Pol/trx. The processivity of Gp90 in DNA replication and its efficiency in single-dNTP incorporation are similar to those of T7 Pol with processive thioredoxin (T7 Pol/trx). The purified Gp90 demonstrated a polymerase activity. The sequence of Gp90 is homologous but not identical to that of other A-family DNA polymerases, such as T7 DNA polymerases (Pol) and DNA Pol I. PaP1 encodes its sole DNA polymerase called Gp90 that was predicted as an A-family DNA polymerase with polymerase and 3'-5' exonuclease activities. This study investigated the function of a novel DNA polymerase of PaP1, which is the lytic phage of Pseudomonas aeruginosa. ![]() However, our knowledge on phage- encoded DNA polymerases remains limited. Most phages contain DNA polymerases, which are essential for DNA replication and propagation in infected host bacteria. Liu, Binyan Gu, Shiling Liang, Nengsong Xiong, Mei Xue, Qizhen Lu, Shuguang Hu, Fuquan Zhang, Huidong Pseudomonas aeruginosa phage PaP1 DNA polymerase is an A-family DNA polymerase demonstrating ss DNA and ds DNA 3'-5' exonuclease activity.
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